Catalase has intrinsic fluorescence due to its tryptophan and tyrosine residues, but I wonder if it can fluoresce at a longer wavelength, due to other residues (such as NADPH). I haven't seen anything reported other than the peak of tryptophan. I analyzed pure catalase in the lab and I saw a weak peak around Ex/Em ~ 310/420nm, but I can't find anything related to that in the literature.
Thank you
Dear Bojidarka,
Are you saying that the weak peak I'm detecting is also due to Trp? could you please provide a reference where I can read more about it?
Thank you very much,
Vanessa
Catalase contains heme. Perhaps that is the source of the additional spectral feature. It could also be a minor contaminant. It looks like a very minor feature compared to the aromatic amino acid fluorescence. NADPH fluorescence excitation and emission peaks are at 340/460 nm.
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