Can catalase fluoresce at Ex/Em ~ 310/420nm?

Catalase has intrinsic fluorescence due to its tryptophan and tyrosine residues, but I wonder if it can fluoresce at a longer wavelength, due to other residues (such as NADPH). I haven't seen...

03 April 2018 9,629 3 View

CDCFDA emission in dead cells?

I treated pertussis cells with ethanol and then I analyzed them in a flow cytometer after staining with cDCFDA. I would like to know why I'm getting such a strong fluorescence signal with cDCFDA...

08 September 2017 647 3 View

DCFDA for ROS detection?

When I worked with DCFDA in B. pertussis, sometimes I see a yellow color after one hour of incubation (protected from light at room temperature) and sometimes not. For carboxy-DCFDA, I don't see...

04 May 2017 4,131 5 View

DCFDA reaction with H2O2?

Could somebody please explain why I'm obtaining fluorescence signal when I combine DCFDA and H2O2 in buffer? My understanding is that DCFDA must first react with esterases to eliminate the acetyl...

01 February 2017 5,925 3 View

Degradation of DNA and RNA in bacteria?

I understand that degradation of DNA doesn't happen so quickly as RNA. Somebody could please explain why and how this can affect flow cytometric analysis? I haven't seen any protocol for dyes that...

06 July 2016 1,939 0 View

Why alcohol fixation gives lower CV than paraformaldehyde (PFA) cell fixation?

In the literature it is reported that PFA gives poor quality profiles (high CVs) in comparison to ethanol fixation. Could somebody explain why? Thank you!

04 May 2016 2,416 4 View

Doublets discrimination in flow cytometry?

The flow cytometer that I'm using just have the option of width and area for the FL2 detector (yelow/orange fluorescence), but I'm just using the green and the red detectors (FL1 and FL3). For...

02 March 2016 8,104 5 View

Why is BSA used to stabilize solutions?

What is the mechanism in which BSA does so? also, I have seen many protocols for cell fixation/permeabilization where they don't use any stabilizer and just a few where BSA is used with a buffer...

02 March 2016 8,377 6 View

What is the number of events in flow cytometry ?

As I understand the events are the numbers of cells passing through the laser. So, why are there cell counting beads for quantification of cells? having the number the events is not enough? what...

31 December 2015 6,098 7 View

For flow cytometry gating, what is the difference in doing the gating in the flow cytometer and doing it in the software?

When I do the gating in the flow cytometer, does it just record the part that is in the gate? could I do the run without gating in the piece of equipment and then do that in the software?

31 December 2015 6,011 5 View

How to manually calculate p value in ANOVA? what is the equation or formula for it?

How to manually calculate p value in ANOVA? what is the equation or formula for it?

03 March 2021 4,928 5 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

How can I publish a manuscript in free or low cost journal with good H-Index?

Dear all I am going to publish my manuscript which is from my thesis in a good H-Index journal but I found them very expensive(I don't have financial support) based on the exchange currency of my...

28 February 2021 7,800 6 View