Could somebody explain how NADPH (or NADPH-enzyme complex) can be secreted by cells?

02 February 2018 0 6K Report

I'm working with gram negative bacteria, and I would like to know how NADPH could be excreted, or if it is something related to cells lysis.

Thank you,

Badges
Science topic

More Vanessa Zavatti's questions See All

Can catalase fluoresce at Ex/Em ~ 310/420nm?

Catalase has intrinsic fluorescence due to its tryptophan and tyrosine residues, but I wonder if it can fluoresce at a longer wavelength, due to other residues (such as NADPH). I haven't seen...

03 April 2018 9,678 3 View

CDCFDA emission in dead cells?

I treated pertussis cells with ethanol and then I analyzed them in a flow cytometer after staining with cDCFDA. I would like to know why I'm getting such a strong fluorescence signal with cDCFDA...

08 September 2017 698 3 View

DCFDA for ROS detection?

When I worked with DCFDA in B. pertussis, sometimes I see a yellow color after one hour of incubation (protected from light at room temperature) and sometimes not. For carboxy-DCFDA, I don't see...

04 May 2017 4,181 5 View

DCFDA reaction with H2O2?

Could somebody please explain why I'm obtaining fluorescence signal when I combine DCFDA and H2O2 in buffer? My understanding is that DCFDA must first react with esterases to eliminate the acetyl...

01 February 2017 5,976 3 View

Degradation of DNA and RNA in bacteria?

I understand that degradation of DNA doesn't happen so quickly as RNA. Somebody could please explain why and how this can affect flow cytometric analysis? I haven't seen any protocol for dyes that...

06 July 2016 1,999 0 View

Why alcohol fixation gives lower CV than paraformaldehyde (PFA) cell fixation?

In the literature it is reported that PFA gives poor quality profiles (high CVs) in comparison to ethanol fixation. Could somebody explain why? Thank you!

04 May 2016 2,481 4 View

Doublets discrimination in flow cytometry?

The flow cytometer that I'm using just have the option of width and area for the FL2 detector (yelow/orange fluorescence), but I'm just using the green and the red detectors (FL1 and FL3). For...

02 March 2016 8,159 5 View

Why is BSA used to stabilize solutions?

What is the mechanism in which BSA does so? also, I have seen many protocols for cell fixation/permeabilization where they don't use any stabilizer and just a few where BSA is used with a buffer...

02 March 2016 8,424 6 View

What is the number of events in flow cytometry ?

As I understand the events are the numbers of cells passing through the laser. So, why are there cell counting beads for quantification of cells? having the number the events is not enough? what...

31 December 2015 6,169 7 View

For flow cytometry gating, what is the difference in doing the gating in the flow cytometer and doing it in the software?

When I do the gating in the flow cytometer, does it just record the part that is in the gate? could I do the run without gating in the piece of equipment and then do that in the software?

31 December 2015 6,065 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

How to increase citation in Research Gate?

How to enhanced h-index in Research Gate?

04 August 2024 3,368 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View