Sowmya N Rao

4 Questions 3 Answers 0 Followers

Questions related from Sowmya N Rao

Why the aqueous phase is light red/light brown in RNA extraction after centrifugation step?

I am trying to extract RNA from blood sample using TRIzol reagent. I have added TRIzol to blood sample followed by chloroform. But after centrifugation, the aqueous phase is light red/light brown...

10 June 2024 6,742 0 View

What happens to qPCR if extension step is not included?

I am trying to measure telomere length in human DNA samples through qPCR. In the protocol, they have not included extension step. Can anybody explain how a qPCR can work without an extension step?...

01 June 2024 565 2 View

Why I am getting same Ct value in all 10-fold dilutions?

I am trying to make a standard curve with 10-fold dilutions of a standard in qPCR. The initial concentration I am using is 60 picomolar which is diluted 10-folds (10:1) for five more dilutions....

08 May 2024 3,836 0 View

Will centrifugation at low rpm (5000 rpm) will reduce DNA yield (especially DNA fragments)?

I am trying to perform Tunel assay for senescence. DNA fragmentation is one of the characteristics in senescence. I had extracted DNA from scenescent cells and performed Tunel assay. But the RFU...

23 April 2024 4,081 2 View