Sowmya N Rao

2 Questions 3 Answers 0 Followers

Questions related from Sowmya N Rao

What happens to qPCR if extension step is not included?

I am trying to measure telomere length in human DNA samples through qPCR. In the protocol, they have not included extension step. Can anybody explain how a qPCR can work without an extension step?...

01 June 2024 469 2 View

Will centrifugation at low rpm (5000 rpm) will reduce DNA yield (especially DNA fragments)?

I am trying to perform Tunel assay for senescence. DNA fragmentation is one of the characteristics in senescence. I had extracted DNA from scenescent cells and performed Tunel assay. But the RFU...

23 April 2024 4,035 2 View