22 April 2016 5 8K Report

Hello

I want to investigate the effect of high temperature on ROS production in yeast. I have yeast cells which grow at 30 degree as a control and experiment cells grown at high temperature.I used Dihydrorhodamine 123 for staining and then investigated by flowcytometry and fluorescent microscope. Unfortunately, I found high signal for my control cells in flowcytometry and microscope. It’s not reasonable that control cells have more ROS at the normal temperature. I enclosed the results of flowcytometry and fluorescent microscope for my control cells here. I’ll grateful if you could give me helpful suggestions. Thanks in advance.

More as Jebelli's questions See All
Similar questions and discussions