as Jebelli

10 Questions 5 Answers 0 Followers

Questions related from as Jebelli

Why high ROS signal in flowcytometry and fluorescence microscope?

Hello I want to investigate the effect of high temperature on ROS production in yeast. I have yeast cells which grow at 30 degree as a control and experiment cells grown at high temperature.I used...

22 April 2016 8,560 5 View

How can I calculate IC50?

I want to calculate IC50 for MTT data by graghpad. I have two type of MTT data. First, 28 data point and second, 18 data point. In order to analyze in graghpad, I select analyze → XY analyses →...

12 April 2015 7,537 2 View

Which concentrations of drug for gene expression should be used in the MTT assay?

in my MTT assay,IC50 for drug was determined 43 µg/ml (in cell line). I want to analysis the effect of this drug in expression of 4 genes. Which concentrations of drug should I use? Less than IC50...

12 January 2015 9,863 5 View

Serum starvation in cell line treatment?

How long after seeding of cell line, should I do serum starvation or serum 4%? 24 h or less? How long should it last (before treatment) or serum 4%? 24 h or less? i i want to do serum starvation...

06 December 2014 8,987 3 View

How much should the cell density in MTT assay be?

Hi. I have a problem with MTT assay in my cell line. My cell line is B65 and I want to treat these cells with a plant extract. For density set up, I seeded cells in 96 well plate for 3 density:...

03 December 2014 8,430 3 View

What antibiotic for B65 cell line?

hi. I work with B65 cell line (rat neuroblastoma). I do not know to use what type of antibiotic for this cell. I searched in the papers and cell line database, but it is not mentioned there. just...

11 November 2014 947 1 View

Which neuronal cell line is a appropriate selection?

Hi. I want to investigate the effect of an herbal drug for memory genes. I selected PC12 cell line. This cell line differentiates neuron-like cells after NGF treatment. Because the NGF is very...

24 July 2014 4,970 3 View

Can anyone help me with primer design and predicted sequences?

I wanted to design primers for my favorable mRNA (for real time) but for this mRNA in NCBI, there are several predicted transcript variants, in addition to the main mRNA sequencing. Main mRNA...

07 April 2014 5,681 1 View

Any suggested solvents for drug dissolution?

I want to treat my PC12 cell line with one drug, but the drug is insoluble in water and DMSO. I dissolved the drug in DMSO and media, but crystal was formed. What solvent do you suggest? What...

04 February 2014 7,356 4 View

How to extract brucella DNA from human serum?

I want to extract brucella DNA from human serum. I have selected the "salting out" procedure, but I don't have a protocol for this. Could you suggest another procedure?

13 January 2014 2,423 3 View