as Jebelli

6 Questions 4 Answers 0 Followers

Questions related from as Jebelli

Why high ROS signal in flowcytometry and fluorescence microscope?

Hello I want to investigate the effect of high temperature on ROS production in yeast. I have yeast cells which grow at 30 degree as a control and experiment cells grown at high temperature.I used...

22 April 2016 8,490 5 View

How can I calculate IC50?

I want to calculate IC50 for MTT data by graghpad. I have two type of MTT data. First, 28 data point and second, 18 data point. In order to analyze in graghpad, I select analyze → XY analyses →...

12 April 2015 7,461 2 View

Serum starvation in cell line treatment?

How long after seeding of cell line, should I do serum starvation or serum 4%? 24 h or less? How long should it last (before treatment) or serum 4%? 24 h or less? i i want to do serum starvation...

06 December 2014 8,924 3 View

Which neuronal cell line is a appropriate selection?

Hi. I want to investigate the effect of an herbal drug for memory genes. I selected PC12 cell line. This cell line differentiates neuron-like cells after NGF treatment. Because the NGF is very...

24 July 2014 4,910 3 View

Any suggested solvents for drug dissolution?

I want to treat my PC12 cell line with one drug, but the drug is insoluble in water and DMSO. I dissolved the drug in DMSO and media, but crystal was formed. What solvent do you suggest? What...

04 February 2014 7,323 4 View

How to extract brucella DNA from human serum?

I want to extract brucella DNA from human serum. I have selected the "salting out" procedure, but I don't have a protocol for this. Could you suggest another procedure?

13 January 2014 2,380 3 View