6 Questions 4 Answers 0 Followers
Questions related from as Jebelli
Hello I want to investigate the effect of high temperature on ROS production in yeast. I have yeast cells which grow at 30 degree as a control and experiment cells grown at high temperature.I used...
22 April 2016 8,492 5 View
I want to calculate IC50 for MTT data by graghpad. I have two type of MTT data. First, 28 data point and second, 18 data point. In order to analyze in graghpad, I select analyze → XY analyses →...
12 April 2015 7,463 2 View
How long after seeding of cell line, should I do serum starvation or serum 4%? 24 h or less? How long should it last (before treatment) or serum 4%? 24 h or less? i i want to do serum starvation...
06 December 2014 8,925 3 View
Hi. I want to investigate the effect of an herbal drug for memory genes. I selected PC12 cell line. This cell line differentiates neuron-like cells after NGF treatment. Because the NGF is very...
24 July 2014 4,913 3 View
I want to treat my PC12 cell line with one drug, but the drug is insoluble in water and DMSO. I dissolved the drug in DMSO and media, but crystal was formed. What solvent do you suggest? What...
04 February 2014 7,323 4 View
I want to extract brucella DNA from human serum. I have selected the "salting out" procedure, but I don't have a protocol for this. Could you suggest another procedure?
13 January 2014 2,382 3 View