Nai-Kei Wong

4 Questions 214 Answers 0 Followers

Questions related from Nai-Kei Wong

How would you disrupt electrostatic forces in protein-protein interaction assays?

I can think of low pH and high ionic strength (500 mM NaCl, high Mg2+ etc.) Specifically I want to disrupt the bonds between organic phosphates and primary amines.

05 August 2015 6,990 3 View

Any suggestions on a good in vitro (cell) model of hydroxyl radical induction?

While some experts have doubted hydroxyl radical even exists in cells, some say it does but at nearly immeasurably low amounts in extremely short time. In your opinion, what have been the most...

30 June 2015 242 6 View

What heat killing conditions do you use for bacteria?

Often a heat-killed version of the bacteria is used, to contrast with live bacteria in infection assays. There are various different conditions being used in the literature, ranging from 50°C to...

19 March 2015 2,005 10 View

What would be a first (reliable) apoptosis assay before validating by annexin V / PI staining?

We observed some dose dependent cell death in Hela cells after treatment with a potential drug we developed. We want to know what kind of cell death it induces, but without having to dive right...

02 March 2015 6,357 14 View