Why GFP bacterial strain do not fluoresce on some agar media ?

26 October 2023 1 2K Report

Have you already observed that some GFP strains do not fluoresce when they grow on certain agar media ? Do you know why, and what component of the media can interfere with fluorescence expression ?

Thank you

Robert Adolf Brinzer

What promoter are you using? Some promoters can vary in expression depending on compounds in the media eg. sugars or amino acids.

Badges
Science method

More Emilie Macke's questions See All

How should fully standardized expected parameter change (StdYX E.P.C. in Mplus CFA output) be interpreted?

And more specifically, how could I interpret a SEPC of 1.07? It points to a potential cross-loading. The two factors in question are highly correlated.

22 May 2024 5,381 2 View

Pricing as attribute in conjoint absolutely needed?

Hi! I'd like to run a conjoint analysis to understand the utility of each of my attributes for a specific prototype product. I was wandering if I absolutely need to put pricing as an attribute or...

30 November 2023 8,025 1 View

What are the key feature between the species within the Sorex genus skulls and jaws use for skull and jaw identification?

Hello, I am currently working on identifying small North American mammal bones, I can ID down to the genus Sorex using the post-mandibular canal. Are there any key features that can help me...

02 July 2023 1,587 1 View

What can I do with an outlier without excluding the participant on SPSS ?

Hi, I am analyzing data from a survey with various measures. I have already done a selection on the sample to only keep participants who have completed all data (exclusion of any missing data). By...

11 January 2023 3,130 4 View

Does anyone know what are the circular-shaped transparent elements in culture ?

Hello, I'm currently cultivating patients' metastases in a medium supplemented in Penicillin and streptomycin and last week I observed the appearance of those circular transparent components. I...

01 January 2023 964 0 View

I have done an electrophoresis of white colonies from blue white screening. Why are there non-recombinant plasmids bands?

I have done a blue-white screening with E.coli and pUC19 with lambda DNA. Then, I selected some white colonies (and blue colonies as a control) and gel electrophoresed them next to pure pUC19. All...

13 November 2022 399 3 View

In Microbiology, how do you avoid R2B/R2A media with seawater to precipitate ?

I'm trying to prepare R2B/R2A media with seawater to cultivate marine bacteria after autoclaving (110°C) my media. I systematically observe a important precipitate. Despite many tests (in...

30 June 2021 2,715 3 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,698 2 View

Basal granzyme B level in inactivated CD8 T cells - flow cytometry?

Hello everyone, I'm currently analyzing expression of Granzyme B by T cells (CD4, CD8 and gamma delta) and NK cells and I found out that CD8, gamma delta T cells and NK cells express granzyme B...

14 January 2021 3,176 1 View

What is the dilution of the plasma to make an Elisa ghrelin with Phoenix pharmaceuticals kit ?

I have to measure the blood concentration of ghrelin with an Elisa. But in the Phoenix Pharmaceuticals kit I ordered, he asks me to dilute the sample without saying how many times. So has anyone...

29 September 2020 3,194 1 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

How to calculate the molar proportions of the oxides from the XRF analyses?

02 August 2024 2,565 2 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View