I have to measure the blood concentration of ghrelin with an Elisa. But in the Phoenix Pharmaceuticals kit I ordered, he asks me to dilute the sample without saying how many times. So has anyone used this kit before and would they know the dilution to be done or would they know the standard values of the plasma ghrelin concentration of a mouse?
Omotayo Erejuwa
Emilie Doeraene Usually for most kits, the dilution factor will be stated particularly if you are using human serum or plasma. This is very strange. In the absence of that. You can try 2X dilution for 2 samples from different groups (to avoid wasting the kit components) and compare your results with standard.
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