Why does the DNA band get stuck in the sample well?

05 March 2022 0 2K Report

I ran my sample in 8% native page.

As the picture shows, the well 1 is DSPE-PEG2000-DNA, which systhesised by myself, the relative molecular mass is about 8500.

The well 2 is DNA-DBCO, its relative molecular is 5940.1.

And the well 3 is DNA marker.

Why does the DNA band get stuck in the sample well instead of running down? According to the relative molecular weight, the concentration of my gel is low enough.

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