Yuyan Liang

6 Questions 7 Answers 0 Followers

Questions related from Yuyan Liang

Why does the DNA band get stuck in the sample well?

I ran my sample in 8% native page. As the picture shows, the well 1 is DSPE-PEG2000-DNA, which systhesised by myself, the relative molecular mass is about 8500. The well 2 is DNA-DBCO, its...

05 March 2022 1,852 0 View

Why can't I see the band in DNA page electrophoresis?

I performed native page electrophoresis on three single-strand DNA. The concentration of the page was 12%. The relative molecular masses of the three DNA are10300 7884 and 7449. The DNA 1 are...

25 October 2021 6,613 3 View

Hybridization temperature of two complementary single-stranded DNA molecules?

I see reports in the literature that when two DNA single-strands are used to prepare a DNA double-stranded system, they first use a PCR machine to raise the temperature to 95°C, and then slowly...

12 October 2021 4,420 6 View

How to separate liposomes of different size?

I want to separate 800nm and 50nm liposomes by centrifugation. What speed and time should I use?

26 July 2021 9,596 1 View

How can I know the phase transition temperature of the liposomes?

I am preparing liposomes recently via the lipid thin-film hydration method. I want to change the formulation of my liposome. But I don't know if I should change the hydration temperature....

31 March 2021 5,184 4 View

How to prepare liposome microscope samples?

Recently, I am preparing multi-compartment liposomes（MIVs）. I want to observe it with a confocal microscope or a phase contrast fluorescence microscope, but I don’t know how to prepare the...

05 March 2021 8,543 5 View