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Questions related from Sushmita Nayak
Hi ALL, I am trying to stain my paraffin embedded brain tissue sections with DAB stain and a counterstain. My DAB works however my counterstain doesn't work. These are my images attached below. I...
18 October 2024 3,938 3 View
I have human brain tissue samples that I lyse with a nuclear extraction buffer and then the pellet I solubilized in 8M urea. I run samples in Tris-Glycine buffer in premade 4-12% gels. Ran the gel...
05 March 2024 9,774 0 View
I have run these westerns a billion times and something or the other doesn’t work out. For eg, my gel ran straight and fine but when I transfer the gel to my membrane, half of the membrane shows...
28 November 2023 1,553 0 View
I am using premade gels (4-12%) and using Running Buffer Tris Glycine Buffer and Licor SDS loading buffer
27 November 2023 9,047 0 View
Does anyone have experience with siRNA transfection with the Neon on THP1 cells? can anyone share the parameters and when do you add the PMA?
01 October 2021 4,712 3 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
28 February 2021 4,363 2 View
Hi everyone, I am currently trying to differentiate Neural progenitor cells (purchased from a company that differentiated iPSCs to NPC) into cortical neurons as well as hippocampal neurons. I...
17 December 2019 6,240 4 View
Hi I lysed rat hippocampi neurons and ran a western blot for B-III Tubulin protein and I saw protein bands. However, I reprobed the PVDF membrane (after three washes with TBST) with my...
18 April 2019 9,716 10 View
