Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

How to determine the box dimension for umbrella sampling?

I am using Gromacs 2016 to do umbrella sampling of a protein-RNA complex. I am following the Lemkul's tutorial of umbrella sampling, but I couldn't get how the box dimension was determined. For a...

24 April 2019 1,458 7 View

Why does a white patch occur in my 2.5% agarose gel?

I wanted to ask why my 2.5% agarose gel is showing some white patch when observed under UV gel doc? Is it because the Etbr did not uniformly mixed?

13 August 2018 1,178 3 View

How to seggregate RNA bound proteins from a list of pdb files?

I have a dataset of pdb files which are all complex structures. From them, I have to select only those which are protein-RNA complexes. I code in python. I am trying to use a keyword or pattern to...

12 October 2017 9,668 3 View

What is the preffered force field for an idp-RNA complex simulation?

For protein-RNA complex, amber ff14SB works good, but am not sure if this forcefield will be ok to use for a disordered protein complex. Pl. suggest the optimum choice of forcefield and the...

29 November 2016 8,263 6 View

How to find if a pdb file is having complex structure or unbound structure?

I have a set of pdb files which I have to seggregate into monomer, dimer (homo+hetero) and protein-nucleic acid. Kindly help with the keyword to look for, I shall write my code accordingly. Thanks...

01 January 1970 9,006 3 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

What can explain a low expected heterozygosity rate for T. monococcum wild samples (0.09) ?

I conducted an SDS PAGE electrophoresis on wheat wild varieties to separate their glutenins and measure the genetic diversity of my sample. I only ran one electrophoresis. Can a genetic drift...

14 February 2021 3,971 1 View

How to store Fusarium oxysporum for long period in simple methods ?

31 January 2021 1,832 17 View

Is diversity in teaching methods for students important in communicating information?

Is diversity in teaching methods for students important in communicating information?

26 January 2021 3,387 7 View

Corpus linguistics collocate indicative of diversity?

I have a question, as to whether collocations in corpus linguistics can be used to indicate diversity. I have a corpora of media news articles, and I manage to find out the frequency of my target...

19 January 2021 1,446 1 View

I'm curious whether there is any research available on the use of AI related to diversity&inclusion (efforts)?

I'm looking for studies or conceptual papers on the (potential) uses of AI for enhancing diversity and inclusion within organization. The biases concerning the use of (big) data are known and...

18 January 2021 4,222 4 View

Could one suggest a common formula for Penicillium Chrysogenum spore counting by spectroscopy? and also its nm?

I am working with this strain but I do not have the formula for spore counting. please just suggest a spectrophotometric method.

05 January 2021 4,308 3 View

R package for multivariate dispersion as a measure of beta diversity?

Hello, everyone: I've been reading some old and new papers on how to compute beta diversity using incidence or abundance data from several assemblages/communities. For my data, I am interested...

03 January 2021 9,293 1 View

How can we say a species of insects got extinct?

02 January 2021 4,232 10 View

Can forward and reverse sequences of PCR products be entirely different from each other?

Dear All, The forward and reverse sequences obtained from the PCR products are not identical at all. Can I still make the contigs for doing BLASTn? Individually both sequences are showing the...

22 December 2020 5,280 5 View