I have this system where when I add HEPES (10 mM pH 8.0) to a solution of lysozyme (6 µM) and gold ion (500 µM) I am able to get gold nanoparticle formation (based on absorption spectra and color change) But to this buffer if I add tris (10 mM pH 8/.0 ) or KHPO4 (10 mM pH 8.0 ) along with HEPES, I do not see any gold ions reduction. In what way could tris/ KHPO4 be affecting the reducing ability of HEPES?
(HEPES reduces by electron donation from its piperazine ring )
hello amar,
first of all i didn't get why are you using tris or KHPO4 when you are already using HEPES. second thing how did you conclude that HEPES is reducing your gold ions and not the lysozyme, because i think HEPES having -SO3H functional group will be used to stabilize the gold nanoparticles.
however i am not sure about the answer as i did not understand few points in your questions and cant figure out what your actual experiment is about.
Best of luck!!
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