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Questions related from Amar Thaker
I tried to mutate a tyrosine residue to alanine residue for a protein gene in a pET24 vector. The cells successfully transformed and showed the mutation, but I couldn't express the protein. When I...
02 December 2020 3,487 6 View
There are two samples MBP1 and MBP2 which are same samples of high concentration of Maltose Binding Protein (3-4 mg/ml). The other flatter samples is basically same sample but with 1 mM Hydrogen...
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I have this system where when I add HEPES (10 mM pH 8.0) to a solution of lysozyme (6 µM) and gold ion (500 µM) I am able to get gold nanoparticle formation (based on absorption spectra and color...
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These are Maltose Binding Protein crystals and I have to open the seal to make a buffer transfer. The moment I open the wells, within 2 mins the solution turns opaque as shown. Can someone help me...
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Background: I am currently over expressing Maltose Binding Protein from BL21(DE3) strain of E.coli. For purification I pass it through maltose resin affinity column. Before that I add cell lysis...
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