Amar Thaker

5 Questions 4 Answers 0 Followers

Questions related from Amar Thaker

How can Site directed mutagenesis for a point mutation lead to truncation of the gene?

I tried to mutate a tyrosine residue to alanine residue for a protein gene in a pET24 vector. The cells successfully transformed and showed the mutation, but I couldn't express the protein. When I...

02 December 2020 3,488 6 View

Why is there so much peak broadening in MALDI for my protein sample?

There are two samples MBP1 and MBP2 which are same samples of high concentration of Maltose Binding Protein (3-4 mg/ml). The other flatter samples is basically same sample but with 1 mM Hydrogen...

02 May 2019 9,415 3 View

Why does addition of Tris buffer at pH 8.0 inhibits the ability of HEPES buffer at pH 8.0 to reduce gold ions?

I have this system where when I add HEPES (10 mM pH 8.0) to a solution of lysozyme (6 µM) and gold ion (500 µM) I am able to get gold nanoparticle formation (based on absorption spectra and color...

18 October 2018 5,237 1 View

What exactly is happening to my protein crystals when I peel the tape sealing the well ?

These are Maltose Binding Protein crystals and I have to open the seal to make a buffer transfer. The moment I open the wells, within 2 mins the solution turns opaque as shown. Can someone help me...

04 April 2018 5,551 2 View

Can I keep protein extracted from E. coli bacteria (strain BL 21 DE3) in lysis buffer over night after centrifuging and filtering it?

Background: I am currently over expressing Maltose Binding Protein from BL21(DE3) strain of E.coli. For purification I pass it through maltose resin affinity column. Before that I add cell lysis...

01 January 1970 7,150 5 View