I want to study a immunosuppressive gene.
I knock it out in a cancer cell line and transplant it into B6, and compare its growth with WT tumor. I would expect that KO tumor grow slower than the WT. This is an simple experiment.
I saw people use the same cell line but transduced OVA in it. After they transplant the OVA-cells into B6, they do OVA-immunization first, then they compare KO tumor growth and WT tumor.
Why do they use OVA cells and OVA-immunization? The first experiment is much easier and can prove the point.
Here you would like to study immunosuppressive action of a gene. For that you need a strong immune reaction against the tumor so it needs ova immunization and the presence of ova in the tumor cell lines. The proteins in the tumor cells can not induce an immune response against them if they are from murine system and barely immunogenic even if it is a mutated protein. You need an immunodominant antigen to see the effect.
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