Why don't use ampicillin to select transduced mammalian cells?
also, I saw some lenti-vector is Bleo Resistant, is bleo used as bacteria selector or mammalian cells selector?
I'm recently buying some anti-CD3 and anti-CD28 to activate T cells. I also notice some of this clones have been conjugated with fluorescence for the purpose of labeling T cells for flow...
09 October 2019 962 4 View
I have many single-cell sequencing data. I checked my gene of interest in these data. However, all of these single-cell sequencing data showed that my gene has no expression, no matter in immune...
31 December 2018 3,638 3 View
I was doing an over-expression experiment of a gene for a year. It is a surface protein. The biggest trouble is that I can never detect the gene overexpression by western blot. Interestingly, when...
10 November 2018 7,195 3 View
When I first learn cancer biology, my teacher told me that cancer is usually caused by the loss of tumor suppressor , which is like you loss your brake. After it losses the suppressor, it tends...
05 June 2018 8,052 2 View
Are there any mouse cancer cell lines that can form tumor in nude mice but not in normal (immune competent) mice? Please give me some examples. Thanks.
04 May 2018 6,666 2 View
AI seems to be pretty popular these days and there are many applications in our daily life. But are there any AI designed to help our researchers to solve any biology questions?
04 May 2018 4,024 3 View
The two ends of my gene have same restriction sites. And the destination vector also has two same sites. Can I clone the gene directly to the destination vector? I know it's possible that the gene...
03 April 2018 8,318 4 View
According to the website https://www.addgene.org/14883/, it has bleomycin resistance. However, bleomycin can be used both for bacteria selection and cell selection. Then what is the cell...
03 April 2018 3,768 1 View
For CRISPR, transfection or transduction? The CRISPR vectors I bought is lenti vector, so I can either do transfection or transduction. But which is better??
02 March 2018 2,193 4 View
Can I use NEB mutagenesis -- substitution method to clone sgRNA into the vector? I felt it's much easier. Just design the primers containing the sgRNA bp, conduct PCR and do a ligation. And most...
02 March 2018 553 1 View
The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....
27 February 2021 4,450 2 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...
25 February 2021 3,030 1 View
Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...
24 February 2021 9,839 3 View
Hi, I am a third year chemistry undergrad doing my group project in which I need to predict the HPLC retention time of 20 aromatic molecules. to do this we require the chemical properties of these...
24 February 2021 1,384 3 View
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
24 February 2021 3,157 6 View
I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...
20 February 2021 199 2 View
I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...
16 February 2021 8,519 5 View
Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...
09 February 2021 4,994 1 View
Taking a fractional ODE problem as an example. It has come to my attention that if the fractional order parameter is in the interval (0,1], many only presents results for alpha=0.9, 0.99. I saw...
08 February 2021 9,671 3 View