Hello,

I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of insulin. When I plated them post thaw, they had a lot of suspension cells with a few settled colonies. I usually spin down the suspension cells and add back to the flask when I am doing a media change. I have passaged them two times now and I still see the floating suspension clumps. I also did trypan blue staining and there are about 34% live cells in the suspension. I feel it shouldn't take this long to settle down. I have now passaged them twice now and I still see the floating colonies. How long do they take to get to a fully adherent monolayer?

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