How do you prepare your samples? I mean, after add SDS-PAGE and sample buffer to the samples you put the samples in the thermal cycler to 95 degrees for 10 minutes ?

hey, thanks for answering, Yes I put my samples in the thermal cycler  to 95 degrees but for 5 minutes. 

From the picture, I see some disturbance of the dye front. In my experience, this can be caused by the presence of a large amount of lipid in the sample, such as would occur in a sample prepared from a purified membrane preparation.

I don't know, but i took less amount of protein taking from the lysate, and gel ran perfectly. 

Hi Amani,

We have the same problem too and that usually happens when we have high concentration of detergent in our samples. It doesn't affect our protein bands though.