Pedro Paulo Gattai

8 Questions 53 Answers 0 Followers

Questions related from Pedro Paulo Gattai

It is worth submitting a manuscript to the same journal that rejected your paper previously?

If you have a manuscript rejected by a specific journal due to low priority, after respond the reviewer's questions and improve your paper, you will try to submit this to the same journal, or is...

14 February 2018 2,542 9 View

Someone knows if I can use the QIAamp RNA spin column of qiagen to extract miRNA, or to extract total RNA with miRNA?

I have some spin column left over from the kit that originally made to extract total RNA. I want to use these columns to extract microRNA. Is it possible? Has anyone tried it yet?

24 January 2017 2,949 10 View

What is the best endogenous control for qRT-PCR of microRNA from rat kidney samples?

I tried the snoRNA as my endogenous control, but it's not the best control because the samples showed some differences between the CTs.

04 May 2015 1,962 2 View

How does it work with the LNA technology for qRT-PCR?

I have read that the LNA (locked nucleic acid) is used for in situ hybridization, but this technology also works for qRT-PCR?If so, can I only use the primers to the reaction or use the specific...

19 September 2014 8,972 3 View

How many methods for extracting microRNA from tissue are there?

Besides the method by silica column, is there any other method for extracting microRNA from tissue? What would it be?

12 September 2014 7,511 2 View

I made a gel for RNA integrity that showed four bands, what does it mean?

As you can see in the image attached, there are four bands. Three are known (genomic DNA band, 28S and 18S rRNA bands), one is unknown. Do you know what it is?

29 August 2014 3,347 9 View

What the minimum amount of total RNA that I must use to do cDNA by superscript II RT protocol?

I made an extraction of total RNA with mirvana kit of life technologies for read miRNAs from cell samples, occur that the samples stayed with a low concentration (with 200 ng/uL or even less), but...

01 January 1970 8,846 4 View

Could infinity be linked to the concept of eternal in nature?

Could something that does not have an end be related to the concept of eternal in nature? Could you cite without any doubt something that could prove it? If could the infinity to be linked to...

01 January 1970 7,530 2 View