How to decide on annealing temperature for primer pairs in RT-qPCR?

Hello i am trying a pair of new primers. Unfortunately I do not have enough reactions to perform a temperature gradient. My HK primer has Tm of 60.3 (forward) and 60.8 (reverse) and the other...

05 March 2019 186 7 View

Is there a min amount of cells per samples in ROMO1 ELISA?

Hello everyone, I am pretty new to ELISA and I have been thinking of conducting a ROMO1 quantitative sandwich ELISA with NHDFa. I am planning on having three samples in triplicates however in our...

21 November 2018 3,956 2 View

I will buy the LSD1 Inhibitor Screening Assay Kit. Could you suggest me a kit or a brand?

I am planning to buy a LSD1 Inhibitor Screening Assay Kit. I do not have much experience with this. Is it more advantageous to buy the enzyme separately or to take it with the kit? Is there anyone...

29 June 2018 6,105 2 View

How can I prepare AESO as a resin for composite production ?

... in terms of initiator, accelerator, hardener additions and their ratios

16 February 2018 5,454 3 View

Does anyone know where I find suitable protocols for ascorbic acid determination in plant leaves?

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26 December 2013 1,111 4 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Fluo 3-AM in pancreatic beta-cells?

Dear all, I'm trying to understand if a molecule of my interest (capable of inducing glucose-stimulated insulin secretion) determines an increase in intracellular calcium levels in the presence of...

16 February 2021 453 2 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

What is the possible cause of MSCs low growing?

Hello Everyone, We are inexperienced in culture of MSCs trying to grow a sample from BM of a mare. It is the second time that after preparation of new medium (DMEM-F12 with high glucose - 10% Hy...

07 February 2021 9,989 7 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

Need for help. What goes wrong with my differentiatio process of C2C12?

Hello, I'm now working on C2C12 myoblast cell line. Growing the cells was great, but there are some problems during differentiation process. The cells are not differentiating but frequently...

30 January 2021 9,535 2 View