How can I differentiate NCI-H716 cells with matrigel and observe their differentiation?
I am working on some .edf files. I try to extract the frequency distributions from the EEG waves. The thing is, which i can't be sure if it is a problem or not, the most dominant frequency...
28 June 2024 6,897 4 View
Regional directed electric field and full homogenic dispersed electric field
10 June 2024 10,163 11 View
Can I align RNA-seq reads to WES data instead of a reference genome to obtain raw read counts?
18 February 2024 3,201 1 View
In the MTT assay, I read the absorbance values at 570 nm. However, sometimes there are so many deviations in the absorbance values. Hence, I have to take out some (up to 4 values) deviated values...
13 November 2023 4,928 0 View
I am designing primers for my study. I couldn't find answer to my question anywhere. I will try to see the effects of a drug on transcript levels of different genes. I intent to cover all mRNA...
30 September 2023 1,432 3 View
I have a very complicated particle surface including amphiphilic molecules with PEG. I expect to have hydroxyl groups on my surface when my particles are in water. What I try to do is, I try to...
04 September 2023 1,708 2 View
Hi all, I want to learn what you use for operation research undergraduate courses for solutions. I teach both graduate and undergraduate. For graduate courses we use Matlab. However,...
27 February 2023 4,408 3 View
Hello, we applied Epidural Electrical Stimulation to a patient who had a Spinal Cord Injury for the first time in Turkey. As a result of my literature research, no information was given about the...
07 June 2022 6,147 2 View
Hello, does anyone have suggestions/protocols for selective AQP1 and AQP3 inhibition in human RBC? I would also appreciate a lot the protocols for DFP00173 and TC AQP1 for AQP3&1 inhibition...
02 February 2022 8,520 0 View
- why was there constant washing with BSA many times? - why did we use these materials for ICC? What are the alternatives? - why we have seen the actin filaments?
28 November 2021 1,346 3 View
I’m currently reprogramming my fibroblast cells using Simplicon RNA OKSG-cMyc TagRFP Kit and after 13 days, these colony/aggregates started to show up in all of my wells. I have 6 wells, 2 wells...
07 July 2024 9,034 2 View
I'm currently working with mouse uterine organoids and I've seen that many authors perform organoid passaging using mechanical or enzymatic methods to dissolve the Matrigel while breaking the...
01 July 2024 381 2 View
Hello everyone. I cultured isolated PBMCs in Erythroid Expansion Medium for two weeks and then performed electroporation in my expanded PBMCs in order to reprogram them. The first 24h after the...
22 June 2024 346 4 View
I am currently using transwell inserts with the addition of 50 uL of matrigel for an invasion assay with various osteosarcoma cell lines. I have tried various dilutions of matrigel and all result...
17 June 2024 8,695 0 View
I saw the iPSC about a week after holding the stock (12 well and 3 stocks) and there were very few cells attached, and some cells were differentiated. I wonder what the problem is. The plate is...
12 June 2024 6,087 0 View
This shape gradually spreads. Even if you install new cells again, it will change like this at some point. Has anyone experienced the same thing as me? I tried using Matrigel again by aliquot it....
12 June 2024 7,269 5 View
Hello everyone, I am currently working on patient derived organoids from pancreatic cancer (PDAC) patients. I noted that my organoids never grow too much. after 1 month in culture they are...
04 June 2024 646 7 View
Hello! I am trying to stain primary endothelial cells that have been grown on Matrigel to form tubules. I am interested in apical-basal polarity. The cells do form tubules but it is hard to image...
28 May 2024 5,855 4 View
I am using the hanging drop method to develop individual differentiated adipocyte spheroids from the stromal vascular fraction of adipose tissue. With our current timeline they end up 100-300um....
27 May 2024 1,303 1 View
Hi I have following questions regarding iPSC-derived motor neurons: 1. Is it possible to dettached mature motor neurons using accutase and reattach them on coated plates (matrigel, geltrx etc)?...
01 May 2024 3,047 0 View