How to decide on annealing temperature for primer pairs in RT-qPCR?

Hello i am trying a pair of new primers. Unfortunately I do not have enough reactions to perform a temperature gradient. My HK primer has Tm of 60.3 (forward) and 60.8 (reverse) and the other...

05 March 2019 186 7 View

Is there a min amount of cells per samples in ROMO1 ELISA?

Hello everyone, I am pretty new to ELISA and I have been thinking of conducting a ROMO1 quantitative sandwich ELISA with NHDFa. I am planning on having three samples in triplicates however in our...

21 November 2018 3,956 2 View

I will buy the LSD1 Inhibitor Screening Assay Kit. Could you suggest me a kit or a brand?

I am planning to buy a LSD1 Inhibitor Screening Assay Kit. I do not have much experience with this. Is it more advantageous to buy the enzyme separately or to take it with the kit? Is there anyone...

29 June 2018 6,105 2 View

How can I prepare AESO as a resin for composite production ?

... in terms of initiator, accelerator, hardener additions and their ratios

16 February 2018 5,454 3 View

Does anyone know where I find suitable protocols for ascorbic acid determination in plant leaves?

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26 December 2013 1,111 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

How long does it take to get a modeller(for homology modelling) license?

I submitted my e-mail address on modeller website to get a license. The problem is that, the site cannot process my school e-mail (ewhain.net). I submitted my Lab website and school name so that...

25 February 2021 9,054 3 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View

Could anyone help me with a SNP primer desing?

16 February 2021 3,049 3 View

PV system design. 3d modelling and Analysis?

I have been looking for a good cost-effective tool for 3D modelling a glass-glass solar module system. I was initially considering Skelion as a tool but it does not look very promising, a bit...

13 February 2021 8,126 1 View

What is better for analyzing secondary consumer data STATA, R or Python?

I have started working on secondary datasets (e.g. consumer panel data) and was wondering which of these programs is more effective in terms of coding and analyzing secondary data. Any thoughts?

06 February 2021 6,213 3 View

Is it possible to get compass potential paramters from MATERIALS STUDIO 6.0?

Hi all, I wonder whether we can get potential parameters from material studio. I have a licensed version available. If I use polymers and cnt in materials studio to perform MD simulations,...

03 February 2021 7,673 3 View

What new can be added in the field of historical studies to implement more realistic programs?

What new can be added in the field of historical studies to implement more realistic programs in dealing with the events of history and interpreting them with scientific sincerity and competence?

26 January 2021 559 1 View