How to decide on annealing temperature for primer pairs in RT-qPCR?

Hello i am trying a pair of new primers. Unfortunately I do not have enough reactions to perform a temperature gradient. My HK primer has Tm of 60.3 (forward) and 60.8 (reverse) and the other...

05 March 2019 186 7 View

Is there a min amount of cells per samples in ROMO1 ELISA?

Hello everyone, I am pretty new to ELISA and I have been thinking of conducting a ROMO1 quantitative sandwich ELISA with NHDFa. I am planning on having three samples in triplicates however in our...

21 November 2018 3,956 2 View

I will buy the LSD1 Inhibitor Screening Assay Kit. Could you suggest me a kit or a brand?

I am planning to buy a LSD1 Inhibitor Screening Assay Kit. I do not have much experience with this. Is it more advantageous to buy the enzyme separately or to take it with the kit? Is there anyone...

29 June 2018 6,105 2 View

How can I prepare AESO as a resin for composite production ?

... in terms of initiator, accelerator, hardener additions and their ratios

16 February 2018 5,454 3 View

I run a model with panel data using Gretl?

04 October 2017 4,113 8 View

SSR data preperation and analysis

10 August 2017 9,639 4 View

Does anyone know where I find suitable protocols for ascorbic acid determination in plant leaves?

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26 December 2013 1,111 4 View

What is "citation farm"?

After a short search about the term "citation farm", I've found some news and twitter posts which underline the self-citation as the main factor of citation farm. But, to my understanding "farming...

01 January 1970 4,765 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View