Can you suggest a good pipette brand?

Eppendorf is the one I am using..but it is costly. Can you suggest a same quality one with same precision mainly for mol bio work. The pipette need to be available in India

01 February 2018 7,731 5 View

Can anyone from India help me to get DOI membership for my journal ?

Where can I get DOI numbers. What is the procedure for application ?

31 December 2017 5,842 2 View

How to store membrane vesicles without losing activity of any constituents?

04 May 2017 4,988 1 View

How to quantify membrane vesicles?

I am isolating membrane vesicles form bacteria. How I can quantify it and have a specific concentration of vesicles for my study. Is there any easy method to quantify membrane vesicles ?

04 May 2017 2,874 3 View

In what ways we can generate profit from biotech research lab ?

I had plans to start a research lab at Kochi ,Kerala,India. A small scale one of investment bellow 10 lakhs.How can generate profit from the lab

31 December 2016 9,529 1 View

Do you consider the cow urine and dung research in India has some significance ?

I think it something foolish..What is your opinion ?

11 December 2016 8,138 5 View

Why should an Open Access journal take different article processing charges depending on the country ?

We Indians often felt difficult to pay very high APCs of many good international journals. When we convert the dollars to rupee, it will be around 60-90K rupees.Consider many places in the country...

10 November 2016 7,209 5 View

Please suggest some cheap ,Indian made PCR machines ?

I am in search to buy a new cheap PCR machine.Please say the cost too..

10 November 2016 8,702 4 View

How to start a Biotechnology company in India ?

Suggestions regarding all aspects invited

10 November 2016 9,589 1 View

Biotechnological Research inviting new articles ?

Biotechnological Research invites articles for its 3rd volume.Submit before November 30th and publish without any charges.

10 November 2016 7,275 0 View

No title

02 March 2021 3,060 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View