Can you suggest a good pipette brand?

Eppendorf is the one I am using..but it is costly. Can you suggest a same quality one with same precision mainly for mol bio work. The pipette need to be available in India

01 February 2018 7,731 5 View

Can anyone from India help me to get DOI membership for my journal ?

Where can I get DOI numbers. What is the procedure for application ?

31 December 2017 5,842 2 View

How to store membrane vesicles without losing activity of any constituents?

04 May 2017 4,988 1 View

How to quantify membrane vesicles?

I am isolating membrane vesicles form bacteria. How I can quantify it and have a specific concentration of vesicles for my study. Is there any easy method to quantify membrane vesicles ?

04 May 2017 2,874 3 View

In what ways we can generate profit from biotech research lab ?

I had plans to start a research lab at Kochi ,Kerala,India. A small scale one of investment bellow 10 lakhs.How can generate profit from the lab

31 December 2016 9,529 1 View

Do you consider the cow urine and dung research in India has some significance ?

I think it something foolish..What is your opinion ?

11 December 2016 8,138 5 View

Why should an Open Access journal take different article processing charges depending on the country ?

We Indians often felt difficult to pay very high APCs of many good international journals. When we convert the dollars to rupee, it will be around 60-90K rupees.Consider many places in the country...

10 November 2016 7,209 5 View

How to start a Biotechnology company in India ?

Suggestions regarding all aspects invited

10 November 2016 9,589 1 View

Biotechnological Research inviting new articles ?

Biotechnological Research invites articles for its 3rd volume.Submit before November 30th and publish without any charges.

10 November 2016 7,275 0 View

Inviting articles for a special issue on Pharmaceutical Science: Current Challenges ?

09 October 2016 5,524 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View