21 June 2021 1 7K Report

I am attempting to probe for interactors of a membrane protein in E. coli using PL with APEX. I need guidance but do not know of anyone close by who could answer my questions. Anyone here with such wisdom? Thanks!

More Sylvie Ouellette's questions See All
Is there a protocol for extraction of inner-membrane proteins of E. coli that does NOT require ultracentrifugation and is fairly quick?

My protein is embedded in the inner membrane and does not run clearly on gel, but rather appears smeared at the top of the lane since it probably links other debris. Also, the epitope appears to...

01 January 1970 7,078 3 View

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