21 June 2021 1 7K Report

I am attempting to probe for interactors of a membrane protein in E. coli using PL with APEX. I need guidance but do not know of anyone close by who could answer my questions. Anyone here with such wisdom? Thanks!

More Sylvie Ouellette's questions See All
Why am I getting strongest scattered field from a PEC backed finite metasurface array with incident plane wave?

I have a finite array of unit cells, completely backed with PEC. When I try to illuminate it with incident plane wave, I get strong scattered waves from the PEC, apart from scattering at desired...

10 August 2023 7,122 1 View

Technical protocol to proteins extract from rat white adipose tissue?

Does anyone have experience in extracting proteins from rat white adipose tissue? I would like to analyse them in Western Blottingand I couldn't remove all fat cake from samples. Thanks!

29 June 2021 7,992 3 View

Any interest for collaborating with TRE-ALTAMIRA (https://site.tre-altamira.com/hydrology-thematic-exploitation-platform-tep/) ?

Hi, I would suggest to change the project title by InSAR-derived inundation modelling. TRE-ALTAMIRA, a subsidiary of CLS company, is a world expert in processing InSAR scenes for ground motion...

18 September 2018 5,202 1 View

Which dispase would you recommend to separate mouse demis from epidermis for quantifying immune cells by FACS (=keep intact membrane epitopes)?

We are currently trying to set up a protocol to isolate immune cells from mouse epidermis and dermis for analysis of immune cells by FACS. Dispase works very well to separate the 2 layers. However...

07 May 2018 3,820 5 View

Discussing with my colleague Loïc Sauvée, he was wandering what is the real impact of Tweeter. What is its impact for publicizing research?

Other questions on Twitter I ask myself: 1) Is Twitter just noise for research? 2) What is the place for doubt on Twitter when you have only two lines to express yourself? 3) Is Twitter an...

03 November 2017 7,148 0 View

Do you have a conservation protocol with glycerol for aero and anaerobic bacterial strains?

To do otherwise with cryobilles

17 June 2013 8,136 0 View

Is there a protocol for extraction of inner-membrane proteins of E. coli that does NOT require ultracentrifugation and is fairly quick?

My protein is embedded in the inner membrane and does not run clearly on gel, but rather appears smeared at the top of the lane since it probably links other debris. Also, the epitope appears to...

01 January 1970 7,109 3 View

How to simulate unit cell without periodic boundaries?

I am trying to design an array of unit cells, which will be used as coding metasurface. Here we know that in the final array, the adjacent unit cells are not going to be same in dimension and...

01 January 1970 2,192 1 View

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Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...

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Can i use the protease inhibitors during cell membrane vesicle preparation ？?

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