Sylvie Ouellette

2 Questions 2 Answers 0 Followers

Questions related from Sylvie Ouellette

Who has experience using proximity labeling with APEX in bacteria?

I am attempting to probe for interactors of a membrane protein in E. coli using PL with APEX. I need guidance but do not know of anyone close by who could answer my questions. Anyone here with...

21 June 2021 6,678 1 View

Is there a protocol for extraction of inner-membrane proteins of E. coli that does NOT require ultracentrifugation and is fairly quick?

My protein is embedded in the inner membrane and does not run clearly on gel, but rather appears smeared at the top of the lane since it probably links other debris. Also, the epitope appears to...

01 January 1970 7,091 3 View