To do otherwise with cryobilles
I have a finite array of unit cells, completely backed with PEC. When I try to illuminate it with incident plane wave, I get strong scattered waves from the PEC, apart from scattering at desired...
10 August 2023 7,122 1 View
Does anyone have experience in extracting proteins from rat white adipose tissue? I would like to analyse them in Western Blottingand I couldn't remove all fat cake from samples. Thanks!
29 June 2021 7,992 3 View
I am attempting to probe for interactors of a membrane protein in E. coli using PL with APEX. I need guidance but do not know of anyone close by who could answer my questions. Anyone here with...
20 June 2021 6,688 1 View
Hi, I would suggest to change the project title by InSAR-derived inundation modelling. TRE-ALTAMIRA, a subsidiary of CLS company, is a world expert in processing InSAR scenes for ground motion...
18 September 2018 5,202 1 View
We are currently trying to set up a protocol to isolate immune cells from mouse epidermis and dermis for analysis of immune cells by FACS. Dispase works very well to separate the 2 layers. However...
07 May 2018 3,820 5 View
Other questions on Twitter I ask myself: 1) Is Twitter just noise for research? 2) What is the place for doubt on Twitter when you have only two lines to express yourself? 3) Is Twitter an...
03 November 2017 7,148 0 View
My protein is embedded in the inner membrane and does not run clearly on gel, but rather appears smeared at the top of the lane since it probably links other debris. Also, the epitope appears to...
01 January 1970 7,109 3 View
I am trying to design an array of unit cells, which will be used as coding metasurface. Here we know that in the final array, the adjacent unit cells are not going to be same in dimension and...
01 January 1970 2,192 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized - I have few of them listed and can be updated in this...
13 July 2024 2,431 2 View
The recharge of deep sandy groundwater is slow. Does the high level of nitrate promote bacteriological activity knowing that it requires a high level of O2? Thanks
10 July 2024 9,883 5 View
Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...
07 July 2024 7,967 3 View
My study uses C57BL/6J mice and I am required to store portions of the important organs in neutral buffered formalin (NBF). Could I potentially keep those tissues in the formalin for a few weeks...
06 July 2024 8,360 2 View
I am working on an in-vitro model of hypoxia-reoxygenation (H/R) in cardiac cells (H9c2). Due to the unavailability of a hypoxic incubator, I am exploring alternative methods to create hypoxic...
30 June 2024 1,234 2 View
