Dario Pacitti

6 Questions 24 Answers 0 Followers

Questions related from Dario Pacitti

IPSC differentiate after thawing, can anyone help troubleshoot please?

Dear Colleagues, I am having problems with resuscitating cryopreserved iPSCs. Upon thawing I get quite a good survival rate, but all my colonies look completely flat, without smooth borders and...

04 April 2016 6,678 7 View

Is it possible to measure mitochondria number in cells by super resolution microscopy (STORM) using a good mitochondrial fluorescent marker?

Hello All, I would like to ask if anyone know if measuring mitochondria number in cells could be feasible by using STORM microscopy to detect a specific fluorescent mitochondrial marker. If yes is...

11 November 2015 369 5 View

For transgenesis is it better to knock-in a cDNA sequence or a whole gene?

Hello, I am trying to knock-in a gene in the safe harbour region AAVS1 using CRISPR, to correct a loss-of-function mutation of my gene of interest. Could an already spliced mRNA sequence of my GOI...

09 September 2015 8,069 4 View

How important is the orientation of a transgene when using CRISPR to knock-in a cassette in a safe harbour locus?

Hello, I am trying to use CRISPR/Cas9 for the knock-in of a transgene using HDR by targeting the safe harbour locus AAVS1 in human iPSC.Is orientation of the transgene important for its...

09 September 2015 6,821 3 View

Mitomycin C precipitates in PBS solution after storing a -20 degrees. Is this deleterious to cells/compound?

Hi everyone, I have recently purchased mitomycin C from SantaCruz to inactivate MEFs and since insufficient information was provided by the manufacturer on reconstitution and stability I had a...

02 February 2015 3,027 6 View

Does anyone know a company/institute which provide training and wet-lab workshops for CRISPR/Cas9 technologies?

I am struggling to find CRISPR/Cas9 workshops for plasmid design and general training. Does anyone know upcoming training in 2015? Many thanks

10 October 2014 9,730 4 View