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Questions related from Dario Pacitti
Dear Colleagues, I am having problems with resuscitating cryopreserved iPSCs. Upon thawing I get quite a good survival rate, but all my colonies look completely flat, without smooth borders and...
04 April 2016 6,696 7 View
Hello All, I would like to ask if anyone know if measuring mitochondria number in cells could be feasible by using STORM microscopy to detect a specific fluorescent mitochondrial marker. If yes is...
11 November 2015 392 5 View
Hello, I am trying to knock-in a gene in the safe harbour region AAVS1 using CRISPR, to correct a loss-of-function mutation of my gene of interest. Could an already spliced mRNA sequence of my GOI...
09 September 2015 8,090 4 View
Hello, I am trying to use CRISPR/Cas9 for the knock-in of a transgene using HDR by targeting the safe harbour locus AAVS1 in human iPSC.Is orientation of the transgene important for its...
09 September 2015 6,840 3 View
Hi everyone, I have recently purchased mitomycin C from SantaCruz to inactivate MEFs and since insufficient information was provided by the manufacturer on reconstitution and stability I had a...
02 February 2015 3,049 6 View
I am struggling to find CRISPR/Cas9 workshops for plasmid design and general training. Does anyone know upcoming training in 2015? Many thanks
10 October 2014 9,748 4 View