Which tag is better to use for in vivo study in zebrafish; by Western blot or IP? Myc, FLAG, or HA? and how many copies should be used?

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Benjamin Tajer

I have some experience with immunofluorescence, HA and V5 work well, while flag causes non-specific nuclear staining. For Western blot and IP, however, I believe all of these epitopes should work well.

Michel Volovitch

Myc tag (from 3 to 6) works very well in zebrafish embryo (see our paper, Rampon et al, 2015, Development). Can work with monoclonal (9E10) and polyclonal (brighter signal).