Has anybody here have ever faced a problem with expression of m.cherry in zebrafish?

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Jesus Pujol-Marti

Hi Ahmed,

In which plasmid is your marker inserted? Under the control of which promoter? How are you detecting mCherry fluorescence?

Ahmed A. Raslan

Hi Jesus,

thanks very much for your answer and sorry for my late reply. Actually we are using miniTol2 vector that is in pBluscript backbone and we are using Gal4-UAS system the Gal4 under Huc promoter and we use specific filter for mCherry in Zeiss Axiom microscope. Thanks

Time ago I did a similar experiment,

coinjecting HuC;Gal4 + UAS:tdTomatoCAAX (both Tol2kit vectors) + Transposase RNA (20pg each at 1 cell stage) and work very good. May be you have problems in your constructs? If you would like to use these contructs you can contact my previous lab and they can send them to you. I remember we also had a HuC:tdtomatoCAAX tol2kit plasmid ready to inject.

Best

Jesus

thanks very much Jesus actually sometimes I saw mCherry when I coinjected the two construct but in yolk not in spinal cord as its expected from Huc promoter.