Is there any easy protocol for protein and total RNA extraction from single zebrafish embryos for western blot and QPCR?

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Which tag is better to use for in vivo study in zebrafish; by Western blot or IP? Myc, FLAG, or HA? and how many copies should be used?

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Has anybody here have ever faced a problem with expression of m.cherry in zebrafish?

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How to increase a model accuracy ?

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Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

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Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

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Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

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What urea buffer can i use for biotagged protein extraction from streptavidin beads?

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What is the best concentration of cDNA to be used for qPCR?

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Nour Eissa

You can use TRIZOL Protocol. It is the common used one

Ahmed A. Raslan

Thanks for both of You

Domenico Cieri

I would reccomend to extract protein from at least 20 embryos (at 24 hpf). Starting from 10-15 embryos has a very low yeld. Also use a mechanical pestel to grind embryos, this greatly increase the total yeld.