Can anyone give a procedure for NBT/BCIP staining for zebrafish WISH?

More Ahmed A. Raslan's questions See All

Which tag is better to use for in vivo study in zebrafish; by Western blot or IP? Myc, FLAG, or HA? and how many copies should be used?

I want to do some in vivo study using zebrafish but I don't know which tag is better to use it for WB or IP and which one does not cause a background? Myc, Flag or HA?..Thanks in advance.

01 February 2016 9,947 2 View

Is there any easy protocol for protein and total RNA extraction from single zebrafish embryos for western blot and QPCR?

I am planning to do western blot for zebrafish embryo and also QPCR so I need easy protocol for protein and total RNA extraction. I need to extract total RNA from single embryos Thanks in advance

08 September 2014 5,173 3 View

Has anybody here have ever faced a problem with expression of m.cherry in zebrafish?

When I inject my construct in Zebrafish which has an m.cherry protein as marker I am having problem in m.cherry expressing and I can't see its color.

02 March 2014 4,567 4 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

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What should a Mechanical Engineering PhD scholar focus on during their PhD to enhance their chances of securing a postdoctoral position?

29 July 2024 7,714 4 View

Carmen C Leitch

This is the standard protocol : http://www.nature.com/nprot/journal/v3/n1/full/nprot.2007.514.html

Simone Schindler

Hi Ahmed,

The protocol from the Parichy Lab is pretty good, too.

Best

Simone

http://faculty.washington.edu/dparichy/lab-protocols.html

Ahmed A. Raslan

Thanks very much Carmen and Simone