If scientists got some money, what they will do with it?

If scientists got some money, what they will do with it?

07 March 2018 2,235 13 View

Can MetaPhor agarose compete with polyacrylamide?

I have a procedure involves the using of polyacrylamide, but I don't have it! Can I use MetaPhor instead? What limits the using of MetaPhor instead of polyacrylamide?

01 March 2018 2,866 2 View

What kind of relationship between DNA methylation level and gene expression?

I investigated the relationship between DNA methylation level and gene expression in different plant species under different affects, and I found it negative in general. I get confused when I read...

01 March 2018 4,043 4 View

Why does plant still activating the mechanisms of stress tolerance even if growth factors are adequately secured?

The plant in the attached image is a wild type! It have been grown in pots and all growth conditions were in educate levels. Why does the plant still show symptoms of stress! What is the role of...

26 February 2018 1,677 6 View

Is the gene expression of antioxidants in the different ecotypes is comparable?

We have collected different ecotypes of wild plants species, we intend to evaluate their ability to produce antioxidants by measuring the gene expression of such antioxidant, is that make sens?

25 February 2018 6,061 3 View

What is the suitable kit for extracting DNA from seed tissue?

In many occasions I have problems with DNA extraction from seeds tissue, especially wild types seeds.I tried different protocols, but i still have invalid DNA! Did any body experienced an...

23 February 2018 8,183 4 View

How to evaluate the results of the DNA sequencing?

When you receive DNA sequencing results as in the attached image, is it generally possible to say that the results of the upper row are more confident than the results of the lower in the same image?

28 January 2018 1,229 2 View

Which of the SMAP Passive Soil Moisture products are the best one?

The NASA Soil Moisture Active Passive (SMAP) Produce Soil Moisture Product in HH, VV and dual polarization. Which of these products are the best one? Which of these products are suitable in...

20 July 2017 4,860 3 View

How can I convert Satellite Soil Moisture data (m3/m3) into mm?

30 May 2017 1,064 7 View

Is ther anyone use Preston tube to measure bed shear stress in open channel?

15 January 2017 9,938 5 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Amorphous silica XRD peak at around 10 degree?

21 February 2021 3,417 5 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View