I still have problems with DNA quality caused by phenols, till I integrated two methods, CTAB for homogenizing and silica filter for purification, and it works. Is there an alternative method?
You can try precipitating your sample DNA with ethanol and sodium acetate.
I was wondering if fixed point theorems like Banach's and Brouwer's fixed point theorems are applied anywhere in number theory?
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Hi, this is Ayoob, I have started work on tunneling and propping up and its impact on firm's performance. As it is my thesis-1. To investigate the existence of "Tunneling and Propping up" in...
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If scientists got some money, what they will do with it?
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I have a procedure involves the using of polyacrylamide, but I don't have it! Can I use MetaPhor instead? What limits the using of MetaPhor instead of polyacrylamide?
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I investigated the relationship between DNA methylation level and gene expression in different plant species under different affects, and I found it negative in general. I get confused when I read...
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I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
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I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
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List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
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Purification of gst tagged protein
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I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
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Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...
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What is the heating temperature that I can use to deactivate enzymes in bacterial extract exopolysaccharide without loss of the bioactivity of EPS? Hi I am trying to make the purification (by TCA...
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Good day everyone, Trying to perform protein purification for the first time, gotta buy the whole equipment/reagents. Protein of interest 48 kDa (Antithrombin), expressed from his-tagged plasmid...
16 July 2024 3,692 6 View