If scientists got some money, what they will do with it?

If scientists got some money, what they will do with it?

07 March 2018 2,235 13 View

What kind of relationship between DNA methylation level and gene expression?

I investigated the relationship between DNA methylation level and gene expression in different plant species under different affects, and I found it negative in general. I get confused when I read...

01 March 2018 4,043 4 View

Why does plant still activating the mechanisms of stress tolerance even if growth factors are adequately secured?

The plant in the attached image is a wild type! It have been grown in pots and all growth conditions were in educate levels. Why does the plant still show symptoms of stress! What is the role of...

26 February 2018 1,677 6 View

Is the gene expression of antioxidants in the different ecotypes is comparable?

We have collected different ecotypes of wild plants species, we intend to evaluate their ability to produce antioxidants by measuring the gene expression of such antioxidant, is that make sens?

25 February 2018 6,061 3 View

What is the suitable kit for extracting DNA from seed tissue?

In many occasions I have problems with DNA extraction from seeds tissue, especially wild types seeds.I tried different protocols, but i still have invalid DNA! Did any body experienced an...

23 February 2018 8,183 4 View

How to evaluate the results of the DNA sequencing?

When you receive DNA sequencing results as in the attached image, is it generally possible to say that the results of the upper row are more confident than the results of the lower in the same image?

28 January 2018 1,229 2 View

Which of the SMAP Passive Soil Moisture products are the best one?

The NASA Soil Moisture Active Passive (SMAP) Produce Soil Moisture Product in HH, VV and dual polarization. Which of these products are the best one? Which of these products are suitable in...

20 July 2017 4,860 3 View

How can I convert Satellite Soil Moisture data (m3/m3) into mm?

30 May 2017 1,064 7 View

Is ther anyone use Preston tube to measure bed shear stress in open channel?

15 January 2017 9,938 5 View

How we can calculate soil surface roughness without field measurement?

Mapping surface soil moisture from the SAR images is a demonstrated procedure, but several factors can interfere with the interpretation and must be taken into account. The most important factors...

15 July 2016 3,747 5 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View