What is the most representative state of DNA methylation?

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If scientists got some money, what they will do with it?

07 March 2018 2,235 13 View

Can MetaPhor agarose compete with polyacrylamide?

I have a procedure involves the using of polyacrylamide, but I don't have it! Can I use MetaPhor instead? What limits the using of MetaPhor instead of polyacrylamide?

01 March 2018 2,866 2 View

What kind of relationship between DNA methylation level and gene expression?

I investigated the relationship between DNA methylation level and gene expression in different plant species under different affects, and I found it negative in general. I get confused when I read...

01 March 2018 4,043 4 View

Why does plant still activating the mechanisms of stress tolerance even if growth factors are adequately secured?

The plant in the attached image is a wild type! It have been grown in pots and all growth conditions were in educate levels. Why does the plant still show symptoms of stress! What is the role of...

26 February 2018 1,677 6 View

Is the gene expression of antioxidants in the different ecotypes is comparable?

We have collected different ecotypes of wild plants species, we intend to evaluate their ability to produce antioxidants by measuring the gene expression of such antioxidant, is that make sens?

25 February 2018 6,061 3 View

What is the suitable kit for extracting DNA from seed tissue?

In many occasions I have problems with DNA extraction from seeds tissue, especially wild types seeds.I tried different protocols, but i still have invalid DNA! Did any body experienced an...

23 February 2018 8,183 4 View

How to evaluate the results of the DNA sequencing?

When you receive DNA sequencing results as in the attached image, is it generally possible to say that the results of the upper row are more confident than the results of the lower in the same image?

28 January 2018 1,229 2 View

Which of the SMAP Passive Soil Moisture products are the best one?

The NASA Soil Moisture Active Passive (SMAP) Produce Soil Moisture Product in HH, VV and dual polarization. Which of these products are the best one? Which of these products are suitable in...

20 July 2017 4,860 3 View

How can I convert Satellite Soil Moisture data (m3/m3) into mm?

30 May 2017 1,064 7 View

Is ther anyone use Preston tube to measure bed shear stress in open channel?

15 January 2017 9,938 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View