I have a biodiversity data from two different sampling time (summer and winter) and seven different sites. Each site has three replicate sampling locations. I have previously tested the difference between sites (sampling time grouped together) using ANOSIM and SIMPER, and visualize it using nMDS + 95% ellipse using the vegan package on R.

Now, I am trying to test if there's any difference in results driven by time. I'm imagining the test to compare the difference of each site on there two different sampling times, and then compare the seven sites as a group. Hope I'm making sense, all my knowledge in stats and R are self-learned. Any advice and/or suggestions are much appreciated! Thanks.

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