Does anyone knows about a comercial software to record pressure and temperature during reaction in an Autoclave?

Dear all: Currently I am working with polymerization reaction in a reactor. For this, I need to control temperature, pressure, agitation speed etc. For calculation of rate of polymerization, I...

07 February 2021 355 2 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View

Do I need to correct Sentinel images (_MSIL2A products)?

Hi guys. I have some questions for you. I've seeing a lot of people using sentinel 2A images without atmospheric correction because they say _MSIL2A products are already corrected. However,...

07 December 2020 2,118 2 View

Can anyone help me with a bootstrap resampling problem in TNT?

Greetings, Can anyone help me with a bootstrap resampling problem in TNT? I am currently running an analysis with TNT in a data matrix composed by morphological characters (105 discrete and 25...

23 October 2019 6,645 3 View

Can anyone help me histology TRAP?

Can anyone help me histology TRAP? I'm doing the manufacturer's protocol (SIGMA), and the coloring turns purple. Does anyone know what could be wrong?

09 July 2019 8,894 3 View

Can anyone help me with microplates reading?

I performed assays in microplates for the first time to determine enzymatic activity, but I made a mistake with the reading. The recommended wavelenght for the assay is 550 nm. I used an ELISA...

06 May 2019 3,093 11 View

How to merge two different flowcell of fastq?

Hello, I need to merge two fastq files, which belong to two flowcell, one file has 71 ind and the other 96, so in total they are 167 ind. How can I merge them? I'm analyzing the cyverse data with...

25 November 2018 4,672 3 View

Methods for 3D culture of Hs578T cells?

Hey! I'm currently performing my dissertation using Hs578T cells and I would like to try to perform the mammosphere assay. Has anyone ever worked with this cell line and did this assay? If so,...

18 September 2018 4,619 3 View

What is tRNA level in strain Rosetta (DE3) since it supplies tRNAs for rare codons?

tRNA level in E. coli BL21 strain is ~15% total. I wonder what is tRNA fold increase in strain Rosetta (DE3) since it carries pRARE plasmid.

14 August 2018 5,946 2 View

How to preserve best PBMC samples for performing FACS staining later ON?

08 January 2018 6,965 7 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View