Hi everyone
I am working on MCF7 cell for anti-cancer purpose (MTT assay) and I want to prepare the drug my drug will be 2,4-Di-tert-butylphenol (2,4-DTBP) (which is secondary metabolites extracted usually from natural source like Aspergillus niger and Streptomyces sp)
My question is which solvent is better with this compound is DMSO or Ethanol or Water?
also I want the exactly preparation method like how exactly prepared methods like how much powder and solvent, and if there is centrifuge step or vortex or filter (I want details )
Thank you