Shahad Alsharif

12 Questions 6 Answers 0 Followers

Questions related from Shahad Alsharif

Cell culture for MCF-7?

Hi everyone I do cell culture and every thing was great until I do subculture and check after 7 days the cell look like that fig below ( the side of flask has full growth( 90-95% confluency) and...

21 November 2024 9,299 2 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

Hi everyone I am working on MCF7 cell for anti-cancer purpose (MTT assay) and I want to prepare the drug my drug will be 2,4-Di-tert-butylphenol (2,4-DTBP) (which is secondary metabolites...

29 October 2024 819 0 View

Drug preparation for MTT assay?

I want to prepare the compound 2,4-Di-tert-butylphenol (2,4-DTBP) for an MTT assay on MCF-7 cells I want the drug preparation method, all detail like how much from powder compound and solvent...

29 October 2024 6,275 3 View

Prediction tool in bioinforamtics?

Hello everyone I used pred-HERG 5.0 web server for cardiac toxicity and I want to generate map this like in below (map for other compound) while I was trying for test my compounds one of them...

11 August 2024 267 0 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

04 August 2024 6,127 2 View

Biomarker for breast cancer?

I want a paper include the explain what is the biomarker and how it is work in cancer and listed of molecular biomarker in breast cancer?

22 May 2023 5,031 2 View

What the function of satellite that found in centromere area of chromosome?

what the function of satellite that found in centromere of chromosome????

07 May 2023 1,898 0 View

What the best cytogenetic analysis technique for Fragile X syndrome and what is the difference between molecular diagnosis and cytogenetic analysis?

what the best cytogenetic analysis technique for Fragile X syndrome and what is the difference between molecular diagnosis and cytogenetic analysis?

03 April 2023 2,115 1 View

Docking for protein

hi I work on protein-ligand docking especially for cancer genes. I went to the PDP(protein data bank) website and found hundreds of proteins for the BRCA1 gene, but I was confused about which...

01 January 1970 5,514 3 View

Help explain this results?

I did today tissue culture for mcf7 cell line( breast cancer) and i check my work under microscope i saw this below is this cancer cell or just cells debris?

01 January 1970 3,107 1 View

How to Prepare my Stock Solution?

I want to make stock for my drug (2,4-DTBP) which is the best solvent DMSO (for MTT assay )and i want to maintain 0.05% DMSO how can i prepare that stock? and which is best stock concertation for...

01 January 1970 7,029 1 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

Hi everyone I am working on MCF7 cell for anti-cancer purpose and I want to prepare the drug (secondary metabolites extracted from Aspergillus niger but these compounds didn’t identify yet) and I...

01 January 1970 4,392 4 View