I start working with microsatellite fragment analysis and now I am looking for a good tool for the analysis of the .fsa data I will obtain. I have heard that Genemapper is a good tool for working with FLA samples. I had a trial version from Bionumerics (where I worked with other Fingerprint data) which seems to me also very good. What is your opinion?
CERVUS is a convenient one I feel but you can try few other softwares too.
Dear Karin
Statistical analysis programmes
Arliquin, Popgene, converters......
Please folwo my page
https://www.researchgate.net/profile/M_El-Sayed2?ev=hdr_xprf&_sg=FN5Mrr-trgkOV-zewRXf1A9MKM7HEB0gOtjfPxfA326c3xkGaIhTV-uNE1KVPaCNX_nR3uWdhor-7f1K3bzgtReY
I recommend GeneMarker is very good program and easy to use, if not Genemapper is also good program
Hi Karin
Genemapper is really powerfull (especailly for different setrs of strains
GeneMarker is OK (but I have not used it myself
Geneious also contains a module for microsatellite scoring.
But the difficulty is to transform raw size into allelic bins (ie. 333.6 into an allele of 333 as any fragment of 332 to 334). You can adjust bin from the comparison of many samples with Genemaper which is really usefull.
Then for genetic analysis , there is a wealth of softwares. But I would recommend R envirnment which offers many softwares.
I am not convinced by what Bionumerics (which is an excellent software) offers for dealing with heterozygotes locu.
Good luck
JL
PS: feel free to contact me for help
Karin Silhavy-Richter, OSIRIS will do fragment analysis with .FSA or .HID files. It's a free NCBI STR and fragment analysis tool with Windows and Macintosh versions:
https://www.ncbi.nlm.nih.gov/osiris/
https://www.ncbi.nlm.nih.gov/osiris/new/
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