I have to get the telomere lenght of a few dozen yeast strains. I've read much about the standard methods but they are mostly only decribed for mammals. The problems I see are:

1. Yeast telomeres are much shorter (350 bp) and some of my mutants even shorter than WT.

2. Telomeric repeats in yeast are irregular.

Does somebody have a nicely working protocol either for qPCR (including good primers) or TRF (including good probes) that gives at least a good estimate of relative TL compared to WT?

Thanks in advance!