Which Rapamycin (Company) do you recommend the most for HEK293 cells treatment?

More Steven Servin's questions See All

AutoGrid and Autodock 4.2.6 window opens for 1 second then closes and there is no output files?

Good day, I am a student trying to work on Autodock for a project regarding Ligand-DNA interaction so i am quite new to molecular docking. i have followed tutorials and did all the steps...

28 July 2024 2,136 4 View

NanoDrop 3300 Error 8008?

Hi everyone, has anyone else experienced an 'Error 8008' with their NanoDrop 3300 (image attached), on a laptop which it previously worked fine on? I have tried uninstalling the software and...

02 June 2024 9,025 3 View

CASTEP: specification of uniform pressure in a non-orthogonal unit cell?

Hello, I want to get confirmation as to the correct way to specify a uniform EXTERNAL_PRESSURE in a non-orthogonal unit cell using the academic CASTEP code (i.e. not using the Materials studio...

29 May 2024 2,878 2 View

Would Total Electron Count (TEC) zones across earth also be part of so-called earth weak magnetic areas, prone to direct CME strikes?

Below is a diagram of TEC zones on earth as a .jpeg file.

25 May 2024 4,051 0 View

Our insulin-tolerance tests have stopped working!?

After lapse of a few months, we recently attempted insulin-tolerance tests in C57BL/6N female mice at 6 months of age. We began, years ago, with Humulin-r at 0.75 U/kg. In our recent tests we...

22 May 2024 3,797 3 View

How can the adoption of genotypic approaches in diagnostic microbiology impact antibiotic resistance prevention?

This question stirs further discussion regarding the potential benefits and challenges associated with integrating genotypic approaches in diagnostic microbiology for antibiotic resistance...

07 April 2024 6,560 2 View

Can you perform an integration from raw acceleration pk-pk post data collection in OROS?

31 March 2024 5,367 1 View

Is there a way to increase the perovskite precusor thickness that DMSO includes at a high ratio?

In the case of the paper, it is said that when the ratio of DMSO to DMF increases, the thickness decreases significantly, and experimentally I feels that way. Can you explain why? And if the...

20 March 2024 5,455 0 View

Is there a solvent that can dissolve perovskite precusor that works better than DMSO?

Is there a solvent that can dissolve perovskite precusor that works better than DMSO? Perovskite precusor contains a lot of Cs and Cl anions

21 February 2024 485 1 View

What caused the swirl like laser spot?

Hello friends, We've been adjusting a laser collimating light path, and we found an interesting phenomenon. We move the collimator along the light axis, we can get a clear and round spot, and...

04 January 2024 5,852 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Saleh Alkarim

Hello

Look at links here will help you

Good Luck

https://academic.oup.com/nar/article/doi/10.1093/nar/gkw1109/2605735/Modulation-of-nonsense-mediated-decay-by-rapamycin

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2568931/

Article PLD1 regulates mTOR signaling and mediates Cdc42 activation of S6K1

Yimin Fang

We used rapamycin from LC lab. Good luck!

Akash Sabarwal

Get it from Sigma Aldrich