Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

Should we reduce the standard unit of length to 0.31951540592 meter ?

The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...

27 February 2021 947 7 View

What metal thin film houses also do annealing?

Could anyone provide names of metal thin film deposition vendors that also do high temperature annealing, in the range of 300-500 C?

27 December 2020 6,137 3 View

Vacuum freeze drying protocol in human skeletal muscle tissue methods, anyone?

I will be freeze-drying small skeletal muscle samples (~60mg) per sample using a Virtis SP Scientific Benchtop Pro Freeze Dryer. I've had difficulty findings a reference protocol to determine the...

21 November 2020 1,423 1 View

Does anyone freeze samples in GeneJET RNA extraction kit lysis buffer?

For the GeneJET RNA purification kit from Thermo Scientific, does anyone know if you can lyse your sample (suspension cells in my case) in the lysis buffer from the kit and then keep the sample at...

18 October 2020 4,993 3 View

Looking for good/reliable betaine assay kits. Does anyone have experience with such a thing?

I am looking to quantify both human serum and skeletal muscle homogenate betaine concentrations via ELISA or colorimetric assay kits. Has anyone had a good experience with any products...

24 September 2020 4,550 3 View

Accurate curvature estimation of CT-scans (image stack)?

We have multiple CT-scans in the format of TIF image stacks. To get the mean curvature we first transform the stack to a triangular mesh (using Marching Cubes). To make the triangles in this mesh...

03 August 2020 7,921 4 View

Input from experts in environmental survey sampling design on draft sampling design decision tree?

Hi. I am trying to help provide some rough advice for selecting amongst alternative sampling designs to measure bird density or abundance. The surveys are short-term in duration such as for impact...

22 January 2020 2,978 3 View

Test for silicone residue from silicone part?

I have a silicone part that will come in contact with food. It will need to pass FDA tests for hexane extraction of silicone residues. Is there a method I can use to remove uncured material and...

09 December 2019 4,246 3 View

Use Betas Estimated in Original Jones Model in Modified Jones Model STATA?

Hello, I can use the following codes in Stata to generate regression according to original Jones model. gen Jones1991_DA=. forval y = 2010(1) 2015{ forval i = 1(1) 6{ display `i' display `y'...

14 October 2019 6,438 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View