Why are my gel pieces not getting destained for in-gel digestion?

My gel pieces are not getting destained. I have kept it for more than 6 hours now. I m using 50mM Ammonium bicarbonate and Acetonitrile in ratio of 7:3.

04 May 2018 1,787 1 View

How to properly store the sds gel pieces for in gel digestion?

I have already cut the gel pieces and would like to perform in gel digestion in future. So I had freeze them in 1% acetic acid. Can I still process those samples?

31 December 2017 3,012 1 View

Is proteomic data analysis in R (using RforProteomics) better than any other proteomic software?

My project involves analyzing proteome of my sample. I have more than 100 samples for analysis. Would proteomic analysis in R help me? Is it better than other softwares?

31 December 2017 6,542 8 View

Why do I get smear on SDS-PAGE?

I am working on snake venom proteins and wanted to resolve it on SDS PAGE. I have attached image of my gel. I am getting a smear. Is there any way to resolve it better?

04 May 2016 9,006 5 View

What is polymorphic marker?

02 March 2016 7,992 2 View

How does DNA get compressed in stacking gel?

I know that it gets sandwiched between glycine and Cl ions But how does it get compress literally?

01 February 2016 7,267 2 View

After running SDS, why do we put our gel in solution of acetic acid and methanol?

I was reading SDS PAGE and methodology it was mentioned, I searched it online but i couldn't find the answer.

01 February 2016 453 5 View

Can anyone give me examples of gene-lists and gene-sets used in GSEA?

I wanted to understand gene set enrichment analysis. For that I need some practical examples of gene-lists and gene-set used in this technique

09 October 2015 7,509 4 View

How to do northern blotting to check expression of HSP proteins in frog ?

04 May 2015 5,296 3 View

How can I take blood from bull frog?

I have to check effect of temperature on hoplobatracus carcass in peninsular India. I wanted to take blood sample for protein analysis.

03 April 2015 1,778 6 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View