17 Questions 5 Answers 0 Followers
Questions related from Siddharth Bhatia
I have protein samples which I need to identify. I loaded it on SDS PAGE. Now each band may contain two or more proteins. Can I estimate relative abundance of those proteins through IBAQ? If yes,...
06 June 2019 6,114 3 View
My gel pieces are not getting destained. I have kept it for more than 6 hours now. I m using 50mM Ammonium bicarbonate and Acetonitrile in ratio of 7:3.
05 May 2018 1,812 1 View
My project involves analyzing proteome of my sample. I have more than 100 samples for analysis. Would proteomic analysis in R help me? Is it better than other softwares?
01 January 2018 6,564 8 View
I have already cut the gel pieces and would like to perform in gel digestion in future. So I had freeze them in 1% acetic acid. Can I still process those samples?
01 January 2018 3,045 1 View
I am working on snake venom proteins and wanted to resolve it on SDS PAGE. I have attached image of my gel. I am getting a smear. Is there any way to resolve it better?
05 May 2016 9,090 5 View
I was reading an article where they have mentioned alleles of polymorphic markers. they are talking about U2 tandem arrays.
03 March 2016 8,019 2 View
I was reading SDS PAGE and methodology it was mentioned, I searched it online but i couldn't find the answer.
02 February 2016 485 5 View
I know that it gets sandwiched between glycine and Cl ions But how does it get compress literally?
02 February 2016 7,294 2 View
I wanted to understand gene set enrichment analysis. For that I need some practical examples of gene-lists and gene-set used in this technique
10 October 2015 7,536 4 View
I have to quantify expression of HSP proteins in frog. So what could be best possible technique to do that?
05 May 2015 5,319 3 View
I have to check effect of temperature on hoplobatracus carcass in peninsular India. I wanted to take blood sample for protein analysis.
04 April 2015 1,808 6 View
I am performing mass spec data analysis on proteome discoverer. There is a percolator node in the workflow. I wanted to know what does it do?
01 January 1970 5,009 0 View
I am performing in-gel digestion. I wanted to know exact chemistry of the process by which 100% acetonitrile dries up the gel and turn it yellow.
01 January 1970 2,201 5 View
I am performing reverse phase HPLC and using TFA as solvent A. I want to know why m I using it ? why not any other acid ?
01 January 1970 1,477 2 View
I have to perform LD50 experiments. For that I need accurate concentration of venom to be known.
01 January 1970 9,888 6 View
I am performing in gel digestion from gel cut pieces. I am interested to know why do we use 50mM Ammonium bicarbonate as buffer. Why cant we use any other buffer for the experiment?
01 January 1970 4,649 2 View
I am performing in-gel digestion for MS analysis. We use 5% Formic acid to elute the peptides into the solution. I wanted to know the mechanism of this process.
01 January 1970 3,149 2 View
