What is polymorphic marker?

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Which proteomics software is best for performing IBAQ analysis?

I have protein samples which I need to identify. I loaded it on SDS PAGE. Now each band may contain two or more proteins. Can I estimate relative abundance of those proteins through IBAQ? If yes,...

05 June 2019 6,131 3 View

Why are my gel pieces not getting destained for in-gel digestion?

My gel pieces are not getting destained. I have kept it for more than 6 hours now. I m using 50mM Ammonium bicarbonate and Acetonitrile in ratio of 7:3.

04 May 2018 1,828 1 View

How to properly store the sds gel pieces for in gel digestion?

I have already cut the gel pieces and would like to perform in gel digestion in future. So I had freeze them in 1% acetic acid. Can I still process those samples?

31 December 2017 3,059 1 View

Is proteomic data analysis in R (using RforProteomics) better than any other proteomic software?

My project involves analyzing proteome of my sample. I have more than 100 samples for analysis. Would proteomic analysis in R help me? Is it better than other softwares?

31 December 2017 6,581 8 View

Why do I get smear on SDS-PAGE?

I am working on snake venom proteins and wanted to resolve it on SDS PAGE. I have attached image of my gel. I am getting a smear. Is there any way to resolve it better?

04 May 2016 9,119 5 View

How does DNA get compressed in stacking gel?

I know that it gets sandwiched between glycine and Cl ions But how does it get compress literally?

01 February 2016 7,311 2 View

After running SDS, why do we put our gel in solution of acetic acid and methanol?

I was reading SDS PAGE and methodology it was mentioned, I searched it online but i couldn't find the answer.

01 February 2016 506 5 View

Can anyone give me examples of gene-lists and gene-sets used in GSEA?

I wanted to understand gene set enrichment analysis. For that I need some practical examples of gene-lists and gene-set used in this technique

09 October 2015 7,558 4 View

How to do northern blotting to check expression of HSP proteins in frog ?

I have to quantify expression of HSP proteins in frog. So what could be best possible technique to do that?

04 May 2015 5,343 3 View

How can I take blood from bull frog?

I have to check effect of temperature on hoplobatracus carcass in peninsular India. I wanted to take blood sample for protein analysis.

03 April 2015 1,833 6 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

Why can't academics earn the money they deserve?

Only Journals make money from the articles we have worked on for years. Academics do not earn money from their refereeing. Then shouldn't the solution be a system in which academics can earn...

01 August 2024 6,469 6 View

Are there any alternatives to PhenoScanner?

I am currently conducting a mendelian randomization study, and I was attempting to use PhenoScanner to look for potential confounders associated with the selected SNPs (any SNPs significantly...

23 July 2024 5,703 0 View

How do you assign allele numbers and sequence type after amplifying housekeeping genes by PCR for MLST?

How to assign allele numbers and sequence type after amplifying housekeeping genes by PCR for genotyping of S. pneumoniae strains by MLST using bioedit ?

19 July 2024 5,239 2 View

Genotype and allele distribution statistics ?

Hello, how can I do the statistics associated with genotypes and alleles as in the table? thank you very much for your attention.

17 July 2024 7,606 2 View

Chi-square test for allele distribution?

Hello, when calculating the p value for the alleles in the table, how do we place the values in the chi-square test in the four-eyed table? Thank you very much for your attention.

17 July 2024 998 2 View

Am I allowed to upload the full-text of a research article on ResearchGate if my publication is closed-access?

Recently, an article of mine got accepted for publication in a hybrid journal. Our publication was a closed-access one. Thus, readers who are interested in reading the article would have to pay...

10 July 2024 8,527 4 View

ATG in pPICZalfphaA vector: Yes or Not in front of the gene of interest ... ?

Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...

10 July 2024 3,050 5 View

Short communication journals for mini review submission?

Is it possible to submit a mini review in short communication. Please suggest some journals accepting mini review as a short communication.

08 July 2024 8,553 1 View

To what extent should "reading comprehension" be analyzed in an A.R. based on phonological awareness instruction to EFL students using a webtool?

Dear Colleagues and academy, I am conducting an action research study to understand the impact of phonological awareness activities using Quizlet and Boom on the reading comprehension skills of...

02 July 2024 8,480 0 View