I have already cut the gel pieces and would like to perform in gel digestion in future. So I had freeze them in 1% acetic acid. Can I still process those samples?
Interesting question.
I have protein samples which I need to identify. I loaded it on SDS PAGE. Now each band may contain two or more proteins. Can I estimate relative abundance of those proteins through IBAQ? If yes,...
05 June 2019 6,131 3 View
My gel pieces are not getting destained. I have kept it for more than 6 hours now. I m using 50mM Ammonium bicarbonate and Acetonitrile in ratio of 7:3.
04 May 2018 1,828 1 View
My project involves analyzing proteome of my sample. I have more than 100 samples for analysis. Would proteomic analysis in R help me? Is it better than other softwares?
31 December 2017 6,581 8 View
I am working on snake venom proteins and wanted to resolve it on SDS PAGE. I have attached image of my gel. I am getting a smear. Is there any way to resolve it better?
04 May 2016 9,119 5 View
I was reading an article where they have mentioned alleles of polymorphic markers. they are talking about U2 tandem arrays.
02 March 2016 8,035 2 View
I know that it gets sandwiched between glycine and Cl ions But how does it get compress literally?
01 February 2016 7,311 2 View
I was reading SDS PAGE and methodology it was mentioned, I searched it online but i couldn't find the answer.
01 February 2016 506 5 View
I wanted to understand gene set enrichment analysis. For that I need some practical examples of gene-lists and gene-set used in this technique
09 October 2015 7,558 4 View
I have to quantify expression of HSP proteins in frog. So what could be best possible technique to do that?
04 May 2015 5,343 3 View
I have to check effect of temperature on hoplobatracus carcass in peninsular India. I wanted to take blood sample for protein analysis.
03 April 2015 1,833 6 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
Do these peaks in the curve indicate primer dimer or non-specific products?
01 August 2024 7,466 0 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View