I am looking for a IoT simulator ?

I am working on a university project so, I m looking for a good Simulator compatible with LoRa and LoRaWAN protocol to simulate an IoT scenario. Thanks in Advance

20 February 2021 6,352 6 View

What is the Role of temperature In Hummers method for the preparation of graphene oxide?

04 January 2021 5,033 2 View

Any recommendations for a good CRISPR/Cas9 commercial system for functional genomics of fungi/yeast?

I look forward to implying a good CRISPR/Cas9 system with a selective maker for functional genomics of important genes from environmental fungi and yeast.

22 December 2020 1,432 2 View

In the age of Iconic architecture and global trends, can we still design for people? would iconic architecture enhance identities or destroy them?

In historical cities , would iconic buildings add to the character of the cities or change it ? How would we maintain their character , and yet have expressive new buildings ?

03 December 2020 2,740 18 View

How to implement LEACH-C (LEACH centralized) algorithm for CH selection in WSNs?

I am currently working on a project that is based on optimizing cluster head (CH) selection for energy-efficient wireless sensor networks (WSNs) using evolutionary search techniques. For the...

11 October 2020 5,814 3 View

How can we measure absorbance of solution contain nanoparticles by UV light if the solution was colored? Is this possible?

I have greenish-blue solution (from CuSO4 and bacterial supernatent), and I wont to make sure (by UV) if there are nanoparticles in this solution. How can I know, and what is expected range of...

13 July 2020 5,350 6 View

How to train convolutional neural network for enhancing images?

I am new to DL. I am working in Matlab. I want to train the CNN on a set of images to enhance its brightness/contrast. I want to know how can we train and test the CNN for a set of images. any...

27 May 2020 2,459 6 View

What is the suitable statistical analysis to test the relationship between two diseases from 5 different regions?

I have only the total number of cases for each diseases (measured by millions) and I'm trying to find a significance relationship for their coexistence throughout the 5 regions...

24 March 2020 4,397 3 View

What do you reommend as an animal model for anemia?

I am looking for animal models for different types of anemia

23 December 2019 210 6 View

SEM RMSEA Outpot Higher than .08?

Hi all, I tested a factorial model and all the indicators are pretty good except for RMSEA which is .1 Is there a problem in that? Do I have to report it or I can just report other fit...

06 November 2019 8,179 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View