Angular momentum and the Dirac equation - interpretation?

The Dirac equation for a free particle reads H = c*alpha*p + beta*m*c^2 (Sorry for the miserable formatting - how to enter Latex Code or similar?) The commutator [H,L_i] is nonzero for any...

01 February 2021 7,037 1 View

How to measure abundance in an LC-MS/MS experiment?

Is the chromatographic peak a valid measure of a compounds` (relative) abundance in LC-MS data? We have recorded three MS/MS spectra; their retention time falls into a specific chromatographic...

13 January 2021 6,685 6 View

Which collaborative tools for interactively writing R code are out there?

I am searching for tools to collaboratively write R code together (and run it in an online environment). Crucially, the functionality should be similar to Google docs, i.e. all users should be...

26 May 2020 6,846 6 View

What are the greatest insights academic philosophy achieved during the past 100 years?

I'm seeking a justification for the existence of academic philosophical work. My question is about established 'insights': stable advances, which are largely exempted from any further controvery...

25 April 2020 644 9 View

Carbon coating of metal particles?

I am searching for potential project partners (companies) with experience in the carbon coating of metal particles.

03 March 2020 8,208 4 View

Method for the extraction of insect melanin?

Dear colleagues, does anyone know a reliable method for the extraction of melanin (from both cuticle and formed by immune activated phenoloxidase in the body cavity) from whole insects? Thank...

24 May 2019 962 3 View

Does anyone have barnase-resistant E. coli?

Promega is selling those Flexi-System Vectors. However, they come with Barnase as a selection marker and hence cannot simply be transformed into your usual library-competent E. coli strain. Since...

14 January 2019 480 4 View

Can one use 1,5-DAN (97% purity) for MALDI analysis?

1,5-DAN is a fantastic matrix for MALDI ISD (in-souce decay) analysis. Can anyone tell me whether one can use the cheaper 1,5-diaminonaphtalene (97% purity) instead of the much more expensive...

25 November 2018 211 3 View

Detection of pathway activation at the end of an experiment - which methods exist?

Problem: we want to know - at the END of an experiment - if a signalling pathway was activated DURING the experiment. I had read something about a novel technique that allowed this. I think that...

05 November 2018 9,372 3 View

Gaussian16, dG solvation free energy?

Is there any keyword missing to get an idea of the dG solvation free energy of a molecule? # M062X/6-31G* Opt SCRF=(SMD,1stVac,Solvent=water) From what I understand from the gaussian09 user...

25 March 2018 1,196 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View