Christoph Metzendorf

6 Questions 48 Answers 0 Followers

Questions related from Christoph Metzendorf

Are there any proteins that are NOT (or very inefficiently) reabsorbed in the renal tubule?

I was unsuccessful to find anything in the literature about proteins that are not reabsorbed (efficiently) in the renal tubule. Maybe it was the way I searched or it doesn't occur or no one has...

30 June 2021 7,774 1 View

Is it possible to select transgenic zebrafish/embryos by antibiotics (e.g. G418)?

I tried to find out whether its feasible to use the SV40-promoter-NeoR cassette for antibiotic selection of transgenic fish. I was only able to find info about using G418 based selection of...

28 May 2020 3,134 0 View

Does anyone have barnase-resistant E. coli?

Promega is selling those Flexi-System Vectors. However, they come with Barnase as a selection marker and hence cannot simply be transformed into your usual library-competent E. coli strain. Since...

15 January 2019 682 4 View

Detection of pathway activation at the end of an experiment - which methods exist?

Problem: we want to know - at the END of an experiment - if a signalling pathway was activated DURING the experiment. I had read something about a novel technique that allowed this. I think that...

06 November 2018 9,529 3 View

LPS injection (IP) in mice: fixed amount or relative to body weight? Any systematic studies about this?

Some authors use a fixed amount of LPS injected into mice, others adjust the injected LPS dose to the mouse weight. With "high" doses that saturate the immune system it should be irrelevant if a...

19 December 2013 4,449 4 View

Which mouse CCL2 ELISA is working well?

I am looking for a nice price option & a kit that is proven to work well with serum and conditioned medium from cultured cells (bone marrow derived macrophages).

12 December 2012 6,779 8 View